Figure 6. Oro‐facial‐digital syndrome 1 (OFD1) interaction partners are centriole and centriolar satellite proteins, suggesting new components of ciliary basal bodies.

1. Network of ciliopathy complex and closely interacting centrosomal complex. Edge weights represent SVM confidence scores where gray are intracomplex edges and purple are inter‐complex edges. Color of nodes follows Fig 5 conventions.
2. Matrix of AP‐MS evidence supporting both complexes. The matrix shows strong support for interactions within each complex. Bait proteins that are members of either complex are labeled on the left.
3. Experimental validation of ciliary proteins using multi‐ciliated epithelial cells in Xenopus laevis. Localization assays for the three uncharacterized proteins in the OFD1 complex confirm that all three proteins localize to basal bodies at the base of the cilia in a manner similar to known components of the complex. Scale bars: 1 μm. Each image is representative of nine cells from three different embryos.