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. 2017 May 18;14(1):173–180. doi: 10.3892/etm.2017.4477

Figure 3.

Figure 3.

Targeting of IGF-1R by miR-30a-5p. (A) TargetScan software predicated that IGF-1R was a potential target of miR-30a-5p, and that (B) their targeting relationship was evolutionally conserved. (C and D) Luciferase reporter vectors containing WT and MUT IGF-1R 3′UTR were constructed. (E) Luciferase activity was significantly decreased in AGS cells co-transfected with the WT IGF-1R 3′UTR vector and miR-30a-5p mimic, but was unaffected in cells co-transfected with the MUT IGF-1R 3′UTR vector and miR-30a-5p mimic, relative to the control group. (F) Western blot analysis and (G) reverse transcription-quantitative polymerase chain reaction were used to measure the expression of IGF-1R at the protein and mRNA levels, respectively, in AGS cells transfected with miR-NC or miR-30a-5p mimic. Data are presented as mean ± standard deviation of at least three independent experiments **P<0.01 vs. control. IGF-1R, insulin-like growth factor 1 receptor; miR, microRNA; WT, wild-type; MUT, mutant; 3′UTR, 3′ untranslated region; miR-NC, negative control miR; control, non-transfected AGS cells.