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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: Biochem J. 2016 Feb 4;473(8):1027–1035. doi: 10.1042/BJ20150992

FIGURE 2.

FIGURE 2

SR31527 binds directly to KIFC1. (A) The biolayer interferometry (BLI)results confirmed that SR31527 binds KIFC1 (without the existence of microtubules) with a calculated dissociate constant Kd value of 25.4 nM. The experiments were performed and analyzed using the ForteBIO Octet system. (B) The reference 1D 1H NMR spectra of SR31527 in the presence of KIFC1, at 600 MHz and 298 K ( blue-colored) and the corresponding STD-NMR spectrum (×4) (red-colored). SR31527 structure is embedded with its aromatic, methyl and amine hydrogen marked as HAr, HM and HN, respectively, and their corresponding NMR signals assigned.