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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: Biochem J. 2016 Feb 4;473(8):1027–1035. doi: 10.1042/BJ20150992

FIGURE 4.

FIGURE 4

SR31527 induced multiple spindle formation in breast cancer cells. (A) BT549 cells were treated with SR31527 at 50 μM. After 24 h incubation, the cells were fixed, permeabilized and immunolabeled for α- and γ-tubulin for the detection of spindles and centrosomes (green and red fluorescent labeling, respectively). DNA was labeled with NucRed647 (blue). (B) MDA-MB-231, BT549 and MDA-MB-435s were treated with SR31527 at 50 μM for 24 h. The cells were then fixed and stained as described above, and the percentage of multipolar spindles was calculated in each cell line treated with DMSO control or SR31527. All the values are the average of triplicate determinations with the s.d. indicated by error bars. **P<0.01 versus corresponding cancer cells treated with DMSO control.