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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: Methods Cell Biol. 2016 Apr 23;137:173–186. doi: 10.1016/bs.mcb.2016.04.001

Figure 2. Midbody-dependent recruitment of apical plasma membrane proteins determines the site of nascent apical lumen formation.

Figure 2

(A) Schematic model depicting the role of midbody during lumen formation. Red marks apical endosomes and apical plasma membrane. AMIS stands for apical membrane initiation site.

(B-D) MDCK epithelial cells grown in Matrigel/Collagen matrix were fixed and immunostained with anti-cingulin (CGN; AMIS marker, antibody generated in the Prekeris lab) and anti-acetylated microtubule (central spindle marker) antibodies. Panel B show a single image plane. Panels C and D show 3D rendering based on the entire Z-stack (reproduced from [(Li, Mangan, et al., 2014a)] with permission from EMBO Reports).