Figure 2. PDAC cells under chronic acidosis demonstrate reduced aerobic glycolysis.
(A) 3H-glucose uptake and (B) lactate release in S2-013 and Capan-1 cells at control (7.4) and low pH (7.0). (C) LC-MS/MS-based metabolomics analysis of glycolysis metabolites from cells cultured at control and low pH. Data is normalized to that of the cells at control pH (7.4). Error bars represent mean ± S.E.M. from at least three different replicates. A two-tailed Student’s t-test (A–C) was conducted to compare metabolites in cells at low pH relative to control pH with p-values * p<0.05, **p<0.01, *** p<0.001.
