a, Scheme of blastocyst injection protocol to generate chimeric mouse embryos for the analysis of the developmental potential of Dnd−/− ESCs. b, Injected ESCs successfully form chimeric mouse embryos. c, Analysis of the PGCs from chimeric E12.5 embryos. DDX4 served as marker for germ cells and mCherry marked cells derived from injected ESCs. Only Dnd+/+ ESCs were able to give rise to DDX4-positive PGCs. d, Representative FACS plots comparing the ITGB3-positive PGCLC fraction after four days of induction from Dnd+/+ (left panel) or Dnd−/− EpiLCs (right panel). e, Quantification of relative induction efficiency of PGCLCs from Dnd+/+ or Dnd−/− EpiLCs. f, Scatterplot of gene expression levels comparing Dnd1−/− and Dnd1+/+ cells at d0 (left panel), d2 (middle panel), and d4 (right panel) of the PGCLC induction time course. Transcripts are coloured by the q-value of transcript abundance change.