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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Virology. 2017 Apr 24;507:179–191. doi: 10.1016/j.virol.2017.04.021

Figure 3.

Figure 3

Pol II occupancy at the late promoter does not significantly increase upon differentiation but does increase near the polyadenylation site. Chromatin immunoprecipitation (ChIP) was performed using non-specific IgG or antibodies specific for Pol II on chromatin from HPV16 cells grown in monolayer or MC for 24 hours. qPCR was performed on the ChIP products using primers targeting (a) the late promoter/p670 region, (b) the E1 open reading frame, or (c) the E5 open reading frame. Ct values were normalized first to input and then to the IgG control for each experiment. Values represent means of 5 (a), 3 (b), or 4 (c) experiments and bars represent ± one standard error of the mean. *=p<0.05, NS=p>0.05.