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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Virology. 2017 Apr 24;507:179–191. doi: 10.1016/j.virol.2017.04.021

Figure 4.

Figure 4

CDK9 recruitment is upregulated at downstream sites upon differentiation. ChIP was performed using non-specific IgG or antibodies specific for (a) CDK9 or (b) Ser2-phosphorylated Pol II using chromatin from HPV16 cells grown in monolayer or MC for 24 hours. qPCR was performed on the ChIP products using primers targeting p670, E1, or E5 regions. Ct values were normalized first to input and then to the IgG control for each experiment. Values represent means of 3 experiments and bars represent ± one standard error of the mean. *=p<0.05, ***=p<0.01, NS=p>0.05.