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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Virology. 2017 Apr 24;507:179–191. doi: 10.1016/j.virol.2017.04.021

Figure 5.

Figure 5

Late transcription requires CDK9 activity. Cells containing episomal HPV16 were cultured for 24 hrs in monolayer or MC in the presence of various concentrations of flavopiridol (flavo), and total RNAs were subjected to RT-qPCR analysis using primers specific for the E1^E4 spliced mRNA. Fold induction of E1^E4 transcripts upon differentiation in the DMSO-treated sample within each experiment was set to 1. The dotted line represents the IC50. Values represent the average of 6 experiments and bars represent ± one standard error of the mean. ***=P<0.01, NS=p>0.05.