Figure 7.

Cucurbitacin B downregulates the expression of MAPK signalling cascades. Cucurbitacin B exposure markedly inhibited the activation of JNK, ERK1/2 and p38, thus inhibiting the signaling pathway, demonstrated by western blot analysis. MAPK, mitogen activated protein kinases; JNK, c-Jun N-terminal kinases; ERK, extracellular signal-regulated kinases; p-JNK, phosphorylated-JNK; p-ERK, phosphorylated ERK; L1, control; L2, 25 µM cucurbitacin B; L3, 50 µM cucurbitacin B; L4, 100 µM cucurbitacin B.