Table 2.
Optimisation of peptide loading on liposomes.
| Initial peptide added (μg/μmol lipid)a | Final peptide loading (μg/μmol lipid)b, d | nmol peptide/μmol lipidb, d | % Peptide conjugatedc, d |
|---|---|---|---|
| 25 | 21.7 ± 4.5 | 8.5 ± 1.8 | 84.8 ± 17.6 |
| 50 | 17.7 ± 4.6 | 6.9 ± 1.8 | 34.5 ± 8.9 |
| 100 | 21.2 ± 0.9 | 8.3 ± 0.4 | 20.67 ± 0.9 |
a
Liposomes containing 1% mol DSPE-PEG2000-maleimide.
b
Determined by LavaPep Peptide quantification kit.
c
Calculated as a percentage of initial peptide added.
d
Data are represented as mean ± SD.