Figure 2.

(A) The total payload of BAR peptide bound to the surface of avidin-NPs was determined by incubating avidin-NPs with increasing concentrations of BAR-Flc and measuring the NP-associated fluorescence. Binding saturation occurred at an input peptide concentration of 37.1 nmol/mg NPs. Under these conditions, BAR-modified NPs contained 7.42 nmol of BAR/mg NPs. (B) BAR-modified NPs interact with the P. gingivalis cell surface in a dose-dependent manner. Intact P. gingivalis cells were incubated with various amounts of BAR-modified NPs that encapsulated the fluorescent dye C6. Cell-associated fluorescence was then measured after washing to remove unbound NPs.

Abbreviations: NPs, nanoparticles; Flc, 6-carboxyfluorescein; P. gingivalis, Porphyromonas gingivalis; C6, Coumarin 6.