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Expression of surface and pluripotency markers of MSCs at mRNA level. RT-PCR showed the expression of GAPDH, CD29, CD44, CD90, MHC-I and lack of expression of CD34, CD105 and MHC-II. In NTC, dH2O was used for template as a control negative, and in RT sample, RNA was used instead of template to control genomic DNA contamination (A). The expression of SOX-2 gene was observed (B), and no expression of Oct-4 and Nanog was detected (C). M: 100 bp DNA ladder.
