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Immunocytochemical analysis of pluripotency markers (Nanog, SSEA-1, -3 and -4) in equine MSCs at passage 3. The first row belongs to the positive control samples (A, G and J: NTERA2 cell line, and D: P19 cell line); the cells at the second row (B, E, H and K) are the same cells of first row without using secondary antibody, and the third row cells (C, F, I and L) are equine MSCs at P 3. No expression of pluripotency markers was found in equine MSCs.
