Figure 1.

Detection of gene polymorphisms in UGT1A1*6 and UGT1A1*28 quality control genes by DFMH.

Notes: UGT1A1 gene polymorphisms were detected using different fluorescent probes specific for UGT1A1*6 or UGT1A1*28 wild-type or mutant genotypes. The time to reach the specific fluorescence ST was determined for each channel using the fluorescence detector software, Microseq (Tianlong Science and Technology Co., Ltd., Xi’an, China). ΔST was calculated to set the range of ΔST values indicative of each UGT1A1 polymorphism, which could then be used to determine the genotype of each patient. The results were as follows: (A) UGT1A1*6 (G/G), ΔST =−2.9896; (B) UGT1A1*6 (G/A), ΔST =−0.7286; (C) UGT1A1*6 (A/A), ΔST =10.6859; (D) UGT1A1*28 (TA6/6), ΔST =−7.2983; (E) UGT1A1*28 (TA6/7), ΔST =0.8421; and (F) UGT1A1*28 (TA7/7), ΔST =14.8599. The maximum value of ΔST is 5. Therefore, the ΔST values of UGT1A1*6 (G/A), UGT1A1*6 (G/G), and UGT1A1*6 (A/A) were between −2.0657 and 5.000, <−2.0657, and >5.000, respectively, and the ΔST values of UGT1A1*28 (TA6/7), UGT1A1*28 (TA6/6), and UGT1A1*6 (TA7/7) were between −3.5868 and 5.000, <−3.5868, and >5.000, respectively.

Abbreviations: DFMH, digital fluorescence molecular hybridization; ST, signal intensity.