Skip to main content
. 2017 Jul;58(7):3202–3214. doi: 10.1167/iovs.17-21423

Figure 1.

Figure 1

Dysregulated corneal expression of type IV collagens in posterior polymorphous corneal dystrophy associated with a ZEB1 mutation. (A) Stromal (top row), Descemet membrane (DM) (middle row), and corneal endothelial (bottom row) expression of each of the six type IV collagens in a normal cornea (control) and a cornea from an individual with PPCD3 associated with the p.(Pro538Glnfs*10) mutation in ZEB1 (P1). Five independent fields of view encompassing the stroma, DM, and endothelium were captured for the control and PPCD3 corneas. FU/pixel: fluorescence units per pixel; error bars: SEM between the five independent fields. (B) Representative field of view encompassing the stroma, DM, and endothelium captured from a normal cornea and a cornea from P1. Primary antibodies directed against each of the six type IV collagens and a secondary antibody conjugated to a fluorescent moiety (Alexa Fluor 594, red) were used to detect each type IV collagen protein expression. Cornea sections were counterstained with 4′,6-diamidino-2-phenylindole (DAPI), which stained the nuclei blue.