Figure 2.

Dysregulated corneal expression of type IV collagens in posterior polymorphous corneal dystrophy not associated with a ZEB1 mutation. (A) Stromal (top row), Descemet membrane (middle row), and corneal endothelial (bottom row) expression of each of the six type IV collagens in a normal cornea (control) and three corneas from individuals with PPCD not associated with a ZEB1 mutation (P2–P4). Five independent fields of view encompassing the stroma, DM, and endothelium were captured for the control and non-PPCD3 corneas. FU/pixel: fluorescence units per pixel; error bars: SEM between the five independent fields. (B) Representative field of view encompassing the stroma, DM, and endothelium captured from a normal cornea and corneas from P2 to P4. Primary antibodies directed against each of the six type IV collagens and a secondary antibody conjugated to a fluorescent moiety (Alexa Fluor 594, red) were used to detect each type IV collagen protein expression. Cornea sections were counterstained with DAPI, which stained the nuclei blue.