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. 2017 Jun 5;114(25):E4924–E4933. doi: 10.1073/pnas.1615730114

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Fig. S5. — OPB-51602 affects autophagy in glucose-starved cancer cells. (A) Immunoblot analysis of STAT3, pSTAT3, p62, and ubiquitinated proteins in control (siGL3) and p62 knockdown (sip62) cells with and without treatment with OPB-51602 for 16 h in CM. (B) Flow-cytometry analysis of autophagy in DU145 and H358 cells treated with OPB-51602 and OPB-31121 for 6 h in glucose-rich FM. (C) LC3, STAT3, and ubiquitinated proteins in H358 cells treated with OPB-51602, PS-341 (10 μM), and chloroquine (100 μM) for 16 h in CM. (D) STAT3, p62, and ubiquitinated proteins in DU145 (Upper) H358 (Lower) cells treated with OPB-51602 with or without rapamycin (1 μM) for up to 8 h in CM. (E) Distribution of STAT3 and the indicated proteins in DU145 (Upper) and H358 (Lower) cells treated with OPB-51602 and/or rapamycin (1 μM) for 16 h in CM.