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. 2017 Jun 5;114(25):6557–6562. doi: 10.1073/pnas.1619660114

Fig. 5.

Fig. 5.

Growth of cells in the presence of antibiotics. B. thailandensis E264∆hpnN cells expressing the mutant transporter L48F, F117R, D344Y, F541R, W661H, T818A, T819A, or L826F were retarded in growth in liquid LB supplemented with (A) 5 μg/mL chloramphenicol, (B) 1 μg/mL novobiocin, or (C) 1 mg/mL polymyxin B compared with cells expressing wild-type B. thailandensis HpnN. Error bars represent SD (n = 3). *Values of E264∆hpnN/pHERD20 and E264∆hpnN cells expressing the mutant transporters that are significantly lower than that of E264∆hpnN/pHERD20Ωbt_hpnN expressing wild-type HpnN (P < 6 × 10−6 for A, P < 2 × 10−6 for B, and P < 7 × 10−5 for C; Student’s t test).