Timeline study 2. Subjects attended the research unit at 0800 hours and were prepared for the experiments. At 0845 hours subjects received breakfast according to the respective experimental condition. Blood was drawn at 11 time points with a 15-min interval between 0845 hours and 1045 hours, with additional samples at 1130 and 1315 hours. T1–T4 indicates blood samples used for measurement of tryptophan and tyrosine and G1–G9 indicates blood samples used for measurement of glucose, cortisol, ACTH, insulin, testosterone, and SHBG.