Fig. S5.

MiR-211 decline exerts distinct RNA-seq changes from those of Dox administration. (A) In cultured cells (84) TGF-β pathway genes (red), but not cholinergic synapse genes (green), show down-regulation following Dox administration. (B) In the frontal cortex of dTg-211 mice following Dox administration, genes most differentially expressed in the Du et al. (84) dataset [first (red) and five upper (green) percentiles low P value in ref. 84] show similar distribution of up- and down-regulation as all genes. (C) Genes modified in cultured cells following Dox are unchanged in the forebrain of dTg-211 mice administered with Dox. Shown is a scatterplot of per-gene fold change following Dox in both experiments. Dots represent genes: gray, unmodified; green, modified in the mouse forebrain; blue, modified in cells; red, modified in both. Note very few genes along the diagonal, and few genes to be modified in both experiments.