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POVPC analysis by LC MS/MS using MRM. (a). Extracted ion chromatogram (XIC) of PC head-group fragment m/z 184 for POVPC and dPOPC. The oxidised phospholipid, POVPC, eluted at 20.8 min and the internal standard (deuterated POPC) eluted at 30.8 min. (b). Linear dynamic range of oxPLs in serum volumes. Lipids were extracted from 3 to 20 µl serum and analysed in triplicates. 10 µl of serum sample was the ideal volume selected for this study.
