Table 2.

Dissociation constants (K_d) for the interactions between Tiam1 PDZ domain and the peptides listed obtained by fluorescence anisotropy

Peptide	Sequence	Kd (μM)	Fold versus Model a
	P−7-P−6-P−5-P−4-P−3-P−2-P−1-P0
Consensus Model	Ac-SSRKEYYA	112±15b
Syndecanl	dan-TKQEEFYA	26.9±0.9	4.2
Syndecan1 (phos)	dan-TKQEEFYpA	19.3±1.5	5.8
Syndecan1 (A→F)	dan-TKQEEFYF	55.7±3.6	2.0
Caspr4	dan-ENQKEYFF	16.8±5.4	6.7
Caspr4 (F→A)	dan-ENQKEYFA	64.8±5.9	1.7
Neurexin1	Ac-NKDKEYYV	2400±250b	0.05

dan, dansyl moiety; Ac, acetylated.

^aFold versus Model refers to K_d (Model)/K_d (peptide).

^bAffinity determined by NMR titration.