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. 2017 Jun 28;13(6):e1006839. doi: 10.1371/journal.pgen.1006839

Fig 1. RccR and HexR are highly similar and important for wheat rhizosphere colonisation.

Fig 1

1A: Sequence alignment for rccR and hexR from P. fluorescens SBW25. Important amino acid residues for DNA and ligand interactions are marked in blue and red respectively. 1B: 3D homology model of the RccR protein structure. Arg-53 and -56 (blue) are the predicted DNA interaction partners in the helix-turn-helix domain. Ser-139 and -183 (red) are located in the predicted effector binding site. 1C: Rhizosphere colonisation competition assays. The graph shows the ratio of SBW25 WT or ΔrccR/ΔhexR mutants to WT-lacZ colony forming units (CFU) recovered from the rhizospheres of wheat plants seven days post-inoculation. Each dot represents the ratio of CFUs recovered from an individual plant. In each case, differences between SBW25 and ΔrccR or ΔhexR strains are statistically significant (p < 0.05, Mann-Whitney U test).