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. 2017 Jun 28;12(6):e0179585. doi: 10.1371/journal.pone.0179585

Fig 5. The appearance of Acrosin-GFP-positive cells in organ culture.

Fig 5

(A) Fragments of immature testes (P11) from Acr-GFP transgenic littermates were cultivated on agarose blocks. Non-treated (a-c), control lentivirus-injected (d-f) and shR53 lentivirus-injected (g-i) fragments were observed under a fluorescence microscope on day 1 (a, d, g), day 12 (b, e, h) and day 16 (c, f, i) of culture. The photographs beneath (a)-(i) are phase contrast images of each fluorescent field. The scale bar in (i) for (a-i) is 1 mm. (B) Sections from a control lentivirus-injected fragment (a, b) and a shR53 lentivirus-injected (c, d) fragment after 16 days of culture were double-stained with anti-MVH (red) and anti-GFP (green). The scale bar in (d) for (a-d) is 50 μm. (C) Sections from a control lentivirus-injected fragment (a, b) and a shR53 lentivirus-injected (c, d) fragment after 16 days of culture were double-stained with anti-HSC70t (green) and anti-SCP3 (red). The merged images (a, c) are shown together with nuclear DAPI staining (b, d). The scale bar in (d) for (a-d) is 100 μm.