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. 2017 Jun 28;12(6):e0180097. doi: 10.1371/journal.pone.0180097

Fig 2. mTORC2 is an upstream upregulator of SIK3 expression and phosphorylation.

Fig 2

(A) Schematic of the potential interplay between mTOR2 and SIK3. (B) Phosphorylation of HDAC4, AKT, and S6K1 the downstream targets for SIK3, mTOR1 and mTOR2, respectively, were analyzed following stimulation with high salt and IL-17, individually and combined by Western blot. (C) Phosphorylation of HDAC4, AKT, and S6K1 the downstream targets for SIK3, mTOR1 and mTOR2, respectively, were analyzed by Western blot following co-treatment with high salt and IL-17, and Knock down of SIK3, RAPTOR and RICTOR. Scramble siRNA of the original construct were used as controls. (D) Activity of HDAC4, Akt and S6K1 were analyzed by ELISA under various conditions mentioned in (C). Of note, HDAC4 activity was decreased following SIK3 and RICTOR knock down, while, mTOR2 downstream target AKT and mTOR1 downstream target S6K1 activity was decreased following RICTOR and RAPTOR, respectively. All data represented as mean values ± SEM from four independent experiments. Student-t-test performed for statistical analysis (significance p<0.05).