Figure 6.

a) DOX release profile of PAA modified nanoparticles in the neutral PBS solution (pH = 7.4) with and without NIR laser irradiation. b) Relative cell viability (to blank control) of free DOX and DOX loaded PAA modified nanoparticles with or without the NIR irradiation (λ = 808 nm, 1 W cm⁻², 10 min). c) Cell viability of MCF-7 cells exposed to different time (0–20 min) of laser irradiation (NIR 808 nm, 1 W cm⁻²) at DOX concentration of 0.01 µg mL⁻¹. d) LSCM images of MCF-7 cell incubated with DOX loaded PAA modified nanoparticles at different concentration (0–0.1 µg mL⁻¹) for 24 h and then irradiation with NIR. For each panel, images from left to right show the MCF-7 cell nuclei stained by DAPI (blue), DOX fluorescence in cells (red), and overlay of both images (Scale bar: 20 µm).