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. Author manuscript; available in PMC: 2018 Jun 15.
Published in final edited form as: Cancer Res. 2017 May 8;77(12):3181–3193. doi: 10.1158/0008-5472.CAN-16-2797

Figure 2. TWIST1 and HOXA9 are co-expressed in the developing mouse prostate and in autochthonous prostate neoplastic lesions.

Figure 2

(A) Twist1 and Hoxa9 gene expression by qPCR in E15.5-E18.5 UGS and P0, P5 developing prostate (n=3-6 embryos-pups/time point/gene, Pearson correlation coefficient, R2= 0.82). Normalized to expression level at P35. (B) TWIST1 and HOXA9 IHC on serial sections of E17.5 UGS from wild-type mice in cells at the urogenital mesenchyme (UGM)-urogenital epithelium (UGE) interface. UGMi; section of the UGM closest to the UGE interface, UGMe; section of the UGM toward the edge. Graphs represent percentage positive cells (n=4 embryos). (C) TWIST1 and HOXA9 IHC on adjacent serial sections of prostate tissue from probasin (PB)-Cre; Apcflox/flox mice with prostate neoplasia (n=2). Arrows highlight TWIST and HOXA9 co-expression in the same cell-types.