(A) Wdr5 shRNA knockdown in Myc-CaP-TWIST1 cells reduced Hoxa9 expression as shown by qPCR. (B) Myc-CaP-TWIST1 cells with stable knockdown of Wdr5 showed decreased migration and (C) invasion through Matrigel compared to scrambled shRNA control. (D) WDR5 knockout by TALEN in PC3-TWIST1 cells decreased HOXA9 expression. (E) PC3-TWIST1 WDR5 knockout cells had decreased cell migration and (F) invasion through Matrigel cells compared to scrambled shRNA control. (G) Knockdown of Hottip/HOTTIP by siRNA in Myc-CaP-TWIST1 or (J) PC3-TWIST1 cells, respectively, led to decreased Hoxa9/HOXA9 mRNA. (H) Hottip or (K) HOTTIP knockdown led to decreased cell migration and (I) and (L) invasion through Matrigel in Myc-CaP-TWIST1 and PC3-TWIST1 cells, respectively, compared to scrambled control. All were n≥3, Mann-Whitney t-test: *, p< 0.05; **, p< 0.01; ***, p< 0.001, +/-SEM.