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. Author manuscript; available in PMC: 2017 Jun 28.
Published in final edited form as: Reproduction. 2014 Nov 10;149(1):113–123. doi: 10.1530/REP-14-0240

Figure 3. Oxidized spermatozoa displayed lower level of capacitation compared to untreated controls under capacitation conditions.

Figure 3

Spermatozoa previously treated with H2O2 or DA-NONOate were capacitated with FCSu for 3.5h and then, with LPC for 30 minutes to induce the acrosome reaction. Sperm cells were stained with PSA-FITC to visualize the acrosome, and two hundred spermatozoa were observed under fluorescent microscope. Representative blot from 4 other experiments done with different donors (n=5). *Means higher than non-capacitating control (None).