Spermidine reduces the chances of HDAC4 forming complex with MAP1S. (A,B) Immunoblot analyses (A) and quantification (B) of the impact of spermidine on the levels of total or phosphorylated HDAC4 or 14-3-3. (C,D) Immunoblot analyses (C) and quantification (D) of the impact of spermidine on the interaction between HDAC4 and 14-3-3. (E–J) Immunoblot analyses (E,G,I) and quantification (F,H,J) of the impact of spermidine on the interaction between HDAC4 and MAP1S as detected by coimmunoprecipitation with antibody against MAP1S (E–H) or HDAC4 (I,J). Lysates were prepared from HeLa cells carrying endogenous proteins (E,F) or overexpressing MAP1S and HDAC4 (G–J). (K,L) Fluorescent microscopic analyses (K) and quantification of (L) of the colocalization between MAP1S and HDAC4.