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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: J Bone Miner Res. 2017 Apr 28;32(7):1442–1454. doi: 10.1002/jbmr.3133

Figure 2. ISO Increases Vegf-a Bone Levels both In Vivo and In Vitro.

Figure 2

A) ISO (2 hrs, 3mg/kg, IP) increases Vegf-a expression significantly in whole mouse bone, P=.0064 (qPCR, N=3 for ISO, N=5 for PBS). B) ISO increases expression of Vegf-a in MC3T3 mouse osteoblasts, P<.001 (qPCR, N=3). C) ISO increases expression of Vegf-a in primary mouse BMSCs, P=.012 (qPCR, N=6). D) Vegf-a expression does not increase upon ISO treatment in β2ARKO BMSCs (qPCR, N=2). E) ISO-treated CM from BMSC contains higher amounts of mVegf (N=5, p=.0159 via Mann Whitney U Test, *P=<.05, **P=<.01, ***P=<.001).