Fig. 4.

hBMSCs grown on TCP-S surfaces have longer cilia and increased ciliary p-TGFβ RII over four days of culture. (A) Representative images of primary cilia labelled for acetylated α-tubulin (left, green) of hBMSCs cultured on TCP-B and TCP-S surfaces at day 2 and day 4 of culture with corresponding images labelled for p-TGFβ RII (middle, red). Overlaid images were shown at right. Scale bars 2 µm. (B) Cilia prevalence, (C) length and (D) mean ciliary p-TGFβ RII intensity for each group as described above for day 2 and day 4. n = 100–110 cilia measured per group for (C) and (D), n ≥ 5 fields per group with ≥15 cells/field for (B). ^*: p < 0.05, ^***: p < 0.001, TCP-S against TCP-B at correlating time point. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)