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. 2005 Feb 15;19(4):453–461. doi: 10.1101/gad.1263305

Figure 4.

Figure 4.

Differential recruitment of corepressors to the aP2 and GyK genes in adipocytes. (A) ChIP assay of adipocytes transiently transfected with GyK reporter gene as in Figure 1D. (B) Effect of siRNA knockdown of N-CoR and SMRT on basal and rosiglitazone-stimulated expression of GyK-luciferase in 3T3-L1 adipocytes. Lamin siRNA was used as control. (C) ChIP assay of endogenous GyK and aP2 genes in adipocytes ± rosiglitazone treatment. (D) ChIP re-IP using the supernatants and eluates from ChIP performed for the endogenous GyK gene as in C.(E) Western blotting showing the protein levels of N-CoR, SMRT, and HDAC3 in adipocytes and rosiglitazone-treated adipocytes. RAN is used as loading control. (F) Quantitative real-time PCR measurement of GyK and aP2 mRNA expression in adipocytes treated with vehicle, trichostatin A (TSA, 100 nM) (Shang et al. 2002), and sodium butyrate (NaB, 5 mM) for 48 h. (*) p < 0.01 vs. vehicle.