Fig. 4. ER/PR receptor family mRNA sequence analysis. mRNA sequences of ER1 and PR obtained from individual PCR products and derived amino acid sequences were compared. (A) Coding sequences (CDS) of ER1 from CMEC and canine mammary tumor (CMT)12 were aligned with published canine (GenBank No. XM_533454) and human (GenBank No. NM_000125) ER1 mRNA sequences. Inferred amino acid (AA) sequence in bold letters indicates differences between human and canine ER1 sequences. (B) Coding sequences of PR mRNAs from CMEC, CMT9, CMT27, CMT28, CMT47 and CMT119 were aligned with published canine (GenBank No. NM_001003074) and human (GenBank No. M15716) mRNA sequences and the inferred amino acid sequence is noted.