Fig. 5. Changes in nuclear factor kappa B (NF-κB) p65 protein expression in the cytoplasm after porcine circovirus type 2 (PCV2) infection. Western blotting was used to measure cytoplasmic expression of NF-κB p65 at 6, 12, 24, and 48 h in the control, PCV2, MyD88 small interfering (si)RNA, and PCV2 + MyD88 siRNA groups. Expression of β-actin was used as a positive control; ^*p < 0.05, ^**p < 0.01.