Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 28;7:4340. doi: 10.1038/s41598-017-04292-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Purinergic pharmacology and expression of the receptor involved in the electrical response in SKOV-3 cells. (A) A battery of different agonists (all applied at 3 µM) for purinergic receptors was tested in cells held at −40 mV, and the peak current amplitude for each agonist was normalized against that obtained by applying ATP. This resulted in the potency sequence illustrated in graph (B) where data obtained in 50 cells are summarized. (C) The upper panel shows the PCR analysis for P2Y receptors sensitive to ATP/UTP; P2Y₂, P2Y₄ and P2Y₆ receptor subtypes. In SKOV-3 cells, bands with the expected size were obtained for P2Y₂ and P2Y₄ subtypes but not for P2Y₆ (mRNA from HELA cells was used as positive control, and -RT indicates the corresponding reaction control without reverse transcriptase). The images show immunorecognition for P2Y₂ or P2Y₄ receptor proteins in SKOV-3 cells observed by epifluorescence (P2Y receptor in green and nuclei in red labeled with propidium iodide). Scale bar 10 µm. (D) Traces illustrate the effect of AR-C118925, NF340, or MRS2179, antagonists of P2Y₂, P2Y₁ or P2Y₁₁ receptor, respectively. Each set of records corresponds to the current response elicited by ATP (3 µM; black trace) alone or in the presence of an antagonist (gray trace) as indicated. (E) The graph summarizes the results obtained in experiments as in (D). Each bar corresponds to the current amplitude in every condition (n = 16) normalized against the current obtained by applying ATP alone. The graph also shows results of using UTP as agonist and AR-C118925 as antagonist (n = 16; *p < 0.05).