Figure 7.

SKOV-3 migration promoted by ATP was inhibited by K⁺ channel blockers or esiRNA treatment. (A) Bar graph shows SKOV-3 cell migration quantified after a 16-h incubation with 3 different ATP concentrations (0.3, 0.6, or 3 µM). (B) ATP-promoted migration was evaluated in different conditions. Images illustrate cell migration in control condition without added ATP, with 3 µM ATP, and 3 µM ATP co-applied with 10 µM TRAM-34. (C) Bar graph shows cell migration normalized against control. Cells were incubated in different conditions as indicated by + signs in experiments like those shown in (B) (*p < 0.05, experimental condition vs. control without treatment). Note that drugs that activate the K⁺ response promoted migration, while K_Ca3.1 blockers (last 3 bars) in the presence of ATP significantly inhibited migration to basal level, or even below, as in TRAM-34 application (*p < 0.05, compared vs. control without treatment). Migration in the presence of K⁺ blockers was also statistically different with respect to ATP-promoted migration (^#p < 0.05). The data represent means ± S.E.M. of 3 different culture preparations. (D) SKOV-3 cells after 48 h of treatment with esiRNA were assayed for migration promoted either by 3 µM ATP, 3 µM UTP or 300 µM 1-EBIO. For each drug treatment the distinct esiRNA transfection conditions were evaluated as in Fig. 5 and indicated in the set stimulated with ATP (*p < 0.05, experimental condition vs. respective CNT).