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. 2017 Jun 28;7:4339. doi: 10.1038/s41598-017-04511-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of pretreatment on microbial composition in fecal samples stored in GuSCN solution. (a) Microbial composition of fecal samples from 18 healthy individuals at the phylum level. DNA was extracted directly from fecal samples in GuSCN solution by the bead beating method and sequenced. (b) Relative abundance of Bacteroidetes, Firmicutes, Actinobacteria, and Proteobacteria before and after pre-treatment in the DNA extraction procedure is shown. Data are presented as means ± SD (n = 6). An asterisk means statistically significant difference as calculated by using Student’s t test (*p < 0.05, **p < 0.01).