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. 2017 Jun 28;7:4328. doi: 10.1038/s41598-017-04648-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Uptake and degradation of ¹²⁵I- proMMP-2:TIMP-2 complex by rat yolk sac BN16 cells. BN16 cells were incubated with 10 nM ¹²⁵I-proMMP-2:TIMP-2 complex at 4 °C for 2 h to allow surface binding, in the absence (open circles) or presence of 1 μM RAP (closed circles). Some of the cultures were pre-treated with 100 μM chloroquine for 1 h at 37 °C (open triangles). After washing, cells were further incubated with fresh medium pre-warmed at 37 °C, without or with RAP or chloroquine as above. At the indicated times, the amounts of ligand (a) remaining surface-bound (pronase^®-sensitive radioactivity), (b) internalized (pronase^®-resistant radioactivity) and (c) degraded (trichloroacetic acid-soluble radioactivity in conditioned medium) were measured. Values are means ± S.D. of three dishes. This experiment was performed twice with similar results.