FIGURE 1.

FtsH2 is an integral component of EX1-mediated ¹O₂ signaling. RNA- seq-based comparison of gene expression was performed in 5-day-old var2/flu and flu seedlings that were initially grown under continuous light (LL), transferred to dark for 4 hours (4 h D), and then re-exposed to light for 30 (L30′) and 60 min (L60′). (A) Heat map reclassified the expression of previously reported SORGs. After excluding 215 genes induced during dark incubation (a) and 26 genes that either exhibited no expression or were not expressed differentially (b), 168 true ESORGs (c) were identified. (B,C) Venn diagram and heat map revealed that the majority (142 genes; 85%) of ESORGs were downregulated in var2/flu in comparison to flu upon dark-to-light shift. (D) Expression of ¹O₂-specific and EX1/EX2-dependent genes SIB1, WRKY33 and WRKY40 in var2/flu and flu. The average values and standard error of the normalized gene expression (reads per million; rpm) of three independent biological samples obtained by RNA-seq analysis were shown. (E) Validation of RNA-seq using those selected genes (D) by qRT-PCR. Results represent means of three independent biological replicates. Actin2 was used as a control for normalization.