Figure 6.

Determination of the binding domain of signal transducer and activator of transcription 3 (STAT3) for sorcin. (A) Schematics represent the genes encoding the full length and truncated STAT3 molecules. (B) Sorcin interacts with different truncated STAT3 proteins. HEK293T cells were transfected with the full length or truncated STAT3 plasmids or empty vectors and together with pEGFP-sorcin. Twenty-four hours after tansfection, cell lysates were immunoprecipitated by anti-flag monoclonal antibodies. The pellets were subjected to Western Blot analysis using anti-GFP monoclonal antibody. Results are representative of three independent experiments with similar results. (C) A schematic represents the binding domain of STAT3 (aa 581–770 of STAT3) for sorcin.