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. 2017 Jun 29;8:1161. doi: 10.3389/fpls.2017.01161

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Copyright © 2017 Berenguer, Bárány, Solís, Pérez-Pérez, Risueño and Testillano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of BIX-01294 long treatments on microspore-derived embryo production of Brassica napus. (A): Quantification of the embryo production in control and BIX-treated cultures at 1, 2.5, and 5 μM concentrations. Columns represent mean values (±SEM) of the total number of embryos per Petri dish. Different letters on columns indicate significant differences according to ANOVA and Tukey’s tests at p < 0.05. (B–E): Representative plates showing the microspore-derived embryos produced in control (B) and BIX-treated cultures at 1 μM (C), 2.5 μM (D) and 5 μM (E) concentrations, after 30 days. Bars represent 10 mm.