FIGURE 11.

Effects of BIX-01294 long treatments on H3K9 methylation levels and gene expression of BnHKTM SUVR4-like histone methyltransferase and BnLSD1-like demethylase. (A): Quantification of global H3K9 methylation levels, represented as mean optical density (OD) units at 450 nm (±SEM), in control and 2.5 μM BIX-treated embryos of Brassica napus, after 30 days in culture. (B,C): Relative expression of histone methyltransferase BnHKTM SUVR4-like (B) and demethylase BnLSD1-like (C) in control and 2.5 μM BIX-treated embryos, after 30 days in culture. Transcript levels were normalized to control embryo levels. Bars indicate the SEM. Different letters on columns indicate significant differences according to ANOVA and Tukey’s tests at p < 0.05.