FIGURE 12.

Distribution patterns of methylated DNA (5mdC) in microspore proembryos formed in control conditions and short BIX-01294 treatments. 5mdC immunofluorescence and confocal laser scanning microscopy analysis in Brassica napus. Microspore proembryos of control (A–A”’), 1 μM (B–B”’) and 2.5 μM (C–C”’) BIX-01294 treated cultures. (A–C): Nomarsky’s differential interference contrast (DIC) images of the proembryo structure. (A’–C’): DAPI staining of nuclei (blue). (A”–C”): 5mdC immunofluorescence signal (green). (A”’–C”’): Merged images of DAPI (blue) and 5mdC immunofluorescence (green). The same structures are visualized under different microscopy modes in (A–A”’), (B–B”’) and (C–C”’). Bars represent 20 μm.