FIGURE 8.

Effects of BIX-01294 short treatments on microspore embryogenesis induction of Brassica napus and Hordeum vulgare.(A): Quantification of microspore embryogenesis induction and representative micrographs of control and BIX-treated cultures of Brassica napus.(B): Quantification of microspore embryogenesis induction in control and BIX-treated cultures of Hordeum vulgare. Histograms show percentages of proembryos formed in microspore cultures untreated (control) and after short treatment (4 days) with BIX-01294 at different concentrations (1, 2.5, and 5 μM in B. napus, 0.5, 1, and 2.5 μM in H. vulgare); bars in columns indicate the SEM; different letters on columns indicate significant differences according to ANOVA and Tukey’s tests at p < 0.05. Micrographs show proembryos (larger, dense-rounded structures) in representative areas of control and BIX-treated cultures at 1, 2.5, and 5 μM concentrations, in rapeseed. Bars represent 50 μm.