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. 2017 Jan 20;45(7):1758–1769. doi: 10.1007/s10439-017-1797-5

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Design and characterization of the dual chamber microfluidic device with a high resolution membrane. (a) Schematic of the two chambers design. (b) A photograph of the assembled PDMS device with four reservoirs corresponding to inlets and outlets: the porous membrane appears translucent. (c) A top view of a chamber with bonded porous membrane: the border of the porous region on the top can be distinguished by the lower non-porous region, and thus easily aligned with the chamber wall and the circular pillars; one of the 5 pillars appears on the top, in the exact center of the chamber. (d) Top view image of the 1002F membrane with 10% porosity inside device. The porosity is calculated as the ratio of the combined area of the pores to the total area of the patterned region. (e) A cross section of the assembled device: the two chambers and the cylindrical pillars can be visualized as well as the green coloured 1002F membrane (enhanced background fluorescence was used to visualize the membrane in green).