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. 2017 Jun 29;8:730. doi: 10.3389/fimmu.2017.00730

Figure 5.

Figure 5

Levels of Th1 [IFN-γ, interleukin-2 (IL-2), and IL-12] and Th2 (IL-10) cytokines produced by spleen cells of ΔGRA17-immunized mice. Spleen cells from mice were obtained 70 days postimmunization and were stimulated in vitro with soluble Toxoplasma gondii tachyzoite antigen (10 µg/ml). Cell-free supernatants were harvested and evaluated for IL-2, IL-10, IL-12, and INF-γ using ELISA. Significance compared with naïve control mice: ***p < 0.001.