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. 2017 Jun 29;7:279. doi: 10.3389/fcimb.2017.00279

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Copyright © 2017 Jia, Tan, Duan, Pan, Chen, Yin and Jiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the LM4Δhly::E7 strain. (A) Western blot analysis of proteins secreted by LM4Δhly::E7. The culture supernatant of LM4Δhly::E7 was precipitated by TCA and secretory proteins were analyzed using a monoclonal antibody against HPV16 E7 by Western blot. M, protein marker; Lane 1, LM4Δhly::E7; Lane 2, wtLM4. (B) Hemolytic activity of the recombinant strain was detected. The culture supernatants of LM4Δhly::E7 and wtLM4 were two-fold serially diluted, 1% sheep blood cells were added and incubated at 37°C for 1 h.